Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Sources and dilution of antibodies used in the current study.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Recombinant

Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Stereological parameters used for estimating neuronal numbers in the white matter of the lar gibbon. NeuN – neuronal nuclear marker; nNos – neuronal nitric oxide synthase; CR – calretinin.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Marker, Sampling

Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Frequency distribution bar plots of somal volumes of WMICs in the brain of the lar gibbon. (a) Volumes of the soma of WMICs immunoreactive to neuronal nuclear marker (NeuN). Note the median volume is 615.9 μm3, with a range from 63.6 to 3 716.6 μm3. (b) Volumes of the soma of WMICs immunoreactive to neuronal nitric oxide synthase (nNOS). Note the median volume is 817.8 μm3, with a range from 110.6 to 2 899.8 μm3. (c) Volumes of the soma of WMICs immunoreactive to calretinin. Note the median volume is 619.4 μm3, with a range from 56.2 to 1 952.7 μm3. In all plots the bin width = 92.9 μm3.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Marker

Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Photomicrographs of neuronal nitric oxide synthase (nNOS) immunostaining in the rostral portion of the frontal lobe of the lar gibbon showing the distribution of WMICs immunoreactive to nNOS. (a) Moderately magnified image of the superior frontal gyrus (from the region indicated by the b in Fig. 1a), showing the numerous nNOS-immunoreactive WMICs deep to the cerebral cortex (grey matter, GM, white matter, WM). The approximate boundary of the deep border of cortical layer VI and the WM is marked by a dashed line. (b) High magnification image of the cortical/white matter boundary (marked by a dashed line) of the superior frontal gyrus, showing the nNOS-immunoreactive WMICs deep to the cerebral cortex. Arrows in a and b indicate the same neuron for orientation of image location. (c) Moderately magnified image of the fundus of the inferior frontal gyrus (from the region indicated by the d in Fig. 1a), showing the nNOS-immunoreactive WMICs deep to the cerebral cortex (GM) within the WM. The approximate boundary of the deep border of cortical layer VI and the WM is marked by a dashed line. (d) High magnification image of the cortical/white matter boundary (marked by a dashed line) of the fundus of the inferior frontal gyrus, showing the nNOS-immunoreactive WMICs deep to the cerebral cortex within the WM. Arrows in c and d indicate the same neuron for orientation of image location. Scale bar in c = 500 μm and apply to a and c. Scale bar in d = 250 μm and applies to b and d. In all images dorsal is to the top of the image and medial to the left.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Immunostaining

Journal: Nature Communications

Article Title: The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development

doi: 10.1038/s41467-022-28636-7

Figure Lengend Snippet: a , b Unsupervised clustering analysis of the single-cell transcriptomes of Slc18a3 + cells from E12.5 control spinal cord ( Kdm6b f/+ ;Olig2-Cre;mTmG ). 11 c ontrol c lusters, CC0 to CC10, were visualized using tSNE. Cell types were assigned according to the expression of specific marker genes. Each dot represents a single cell and was color-coded based on the cluster identity. c , d Violin plots for selected marker genes in CC0-CC10. e – j tSNE plots to show the expression of the indicated genes in each plot. The color intensity indicates the expression levels of the genes. The tSNE plots in f , g , and i reveal the co-expression pattern of the two genes. f CC8 (V2IN) and CC9 (V3IN) co-express Slc18a3 and Slc17a6 (cyan, Double+), while the remaining clusters express only Slc18a3 (red, Slc18a3+). g CC1 (nbMN) and CC2 (MMC) co-express Isl1 and Lhx3 (cyan, Double+), whereas CC3 (HMC), CC4 (LMCm), and CC6 (PGC-Isl1 + ) express Isl1 only (red, Isl1+). CC8 (V2IN) express Lhx3 only (green, Lhx3+). i CC4 (LMCm) and CC5 (LMCl) co-express Foxp1 and Aldh1a2 (cyan, Double+), whereas CC6 (PGC-Isl1 + ) and CC7 (PGC-Isl1 − ) express only Foxp1 (red, Foxp1+). k Immunohistochemical analyses with Isl1 and Nos1 antibodies in E13.5 wild-type spinal cords at thoracic levels. Nos1 + PGC cells were divided into Isl1 + and Isl1 − subpopulations. Scale bar, 50 μm. 3–6 sections in 3 embryos were analyzed and the representative images were shown.

Article Snippet: Primary antibodies were listed as follow: goat anti-Olig2 (R&D Systems AF2418, 1:500), rabbit anti-Olig2 (Millipore AB9610, 1:1000), guinea pig anti-Mnx1 (Homemade, 1:500), rabbit anti-Isl1 (Abcam ab109517, 1:250), guinea pig anti-Isl1 (Homemade, 1:2000), rabbit anti-Lhx3 (Abcam ab14555, 1:1000), guinea pig anti-Lhx3 (Homemade, 1:250), guinea pig anti-Vsx2 (Homemade, 1:1000) , guinea pig anti-Sox14 (Homemade, 1:500) , rabbit anti-Nkx2-2 (Abcam ab191077, 1:50), rabbit anti-Foxp1 (Abcam ab16645, 1:2000), rabbit anti-Nos1 (Immunostar 24287, 1:1000), rabbit anti-Kdm6b (Novus Biologicals NBP1-06640, 1:100), guinea pig anti-Klf5 , rabbit anti-Mecom (Cell Signaling Technology #2593, 1:500), sheep anti-Zfhx3 (Novus Biologicals AF7384, 1:200), and rabbit anti-Zfhx4 (Sigma HPA023837, 1:200).

Techniques: Expressing, Marker, Immunohistochemical staining

Journal: Nature Communications

Article Title: The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development

doi: 10.1038/s41467-022-28636-7

Figure Lengend Snippet: a Immunohistochemical analyses for Lhx3, FoxP1, and Nos1 and in situ hybridization analyses for Aldh1a2 in E12.5 spinal cords. Foxp1 + MNs, Aldh1a2 + LMC, and Nos1 + PGC were markedly reduced in Mnx1p::Lhx3 transgenic mice, in which Lhx3 expression was expanded to all MNs. Scale bars, 50 μm. b Immunohistochemical analysis with Kdm6b antibody. Kdm6b protein was detected at E11.5 and substantially removed by E12.5 in Kdm6b -cKO; Isl1-Cre mice. Scale bars, 50 μm. c–g Immunohistochemical analyses for Lhx3, FoxP1, Mnx1, and Isl1, in situ hybridization analyses for Aldh1a2, Slc18a3, Klf5, and Kcnip4 in E13.5 spinal cords, and the quantification of specific marker-labeled MNs per 12 μm thick section. In Kdm6b -cKO; Isl1-Cre mice, Lhx3 + and Mnx1 + MNs were significantly reduced, but neither Foxp1 + nor Isl1 + MNs changed significantly. Scale bars, 50 μm. The error bars represent the standard deviation of the mean. ** p < 0.01; ns non-significant in the two-tailed Student’s t -test. n = 3–6 mice per genotype and 9 slices per genotype. Source data are provided as a Source Data file. The exact p -values are as follows. Lhx3, brachial, 1.44 × 10 −8 ; thoracic, 8.05 × 10 −10 . Foxp1, brachial, 0.68; thoracic, 0.94. Mnx1, brachial, 1.79×10 −9 ; thoracic, 2.36 × 10 −7 . Isl1, brachial, 0.55; thoracic, 0.76.

Article Snippet: Primary antibodies were listed as follow: goat anti-Olig2 (R&D Systems AF2418, 1:500), rabbit anti-Olig2 (Millipore AB9610, 1:1000), guinea pig anti-Mnx1 (Homemade, 1:500), rabbit anti-Isl1 (Abcam ab109517, 1:250), guinea pig anti-Isl1 (Homemade, 1:2000), rabbit anti-Lhx3 (Abcam ab14555, 1:1000), guinea pig anti-Lhx3 (Homemade, 1:250), guinea pig anti-Vsx2 (Homemade, 1:1000) , guinea pig anti-Sox14 (Homemade, 1:500) , rabbit anti-Nkx2-2 (Abcam ab191077, 1:50), rabbit anti-Foxp1 (Abcam ab16645, 1:2000), rabbit anti-Nos1 (Immunostar 24287, 1:1000), rabbit anti-Kdm6b (Novus Biologicals NBP1-06640, 1:100), guinea pig anti-Klf5 , rabbit anti-Mecom (Cell Signaling Technology #2593, 1:500), sheep anti-Zfhx3 (Novus Biologicals AF7384, 1:200), and rabbit anti-Zfhx4 (Sigma HPA023837, 1:200).

Techniques: Immunohistochemical staining, In Situ Hybridization, Transgenic Assay, Expressing, Marker, Labeling, Standard Deviation, Two Tailed Test

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury

doi: 10.1016/j.jcmgh.2022.10.018

Figure Lengend Snippet: Antibodies Used and Their Concentrations

Article Snippet: Rabbit anti-nNOS , Genetex (GTX133403) , IF (1:200).

Techniques: Concentration Assay